Uchiyama, Keiji Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hara, Hideyuki Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Chida, Junji Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Pasiana, Agriani Dini Tokushima University
Imamura, Morikazu University of Miyazaki
Mori, Tsuyoshi University of Miyazaki
Takatsuki, Hanae University of Miyazaki
Atarashi, Ryuichiro University of Miyazaki
Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrPSc. PrPSc is produced through conformational conversion of the cellular isoform of prion protein, PrPC, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrPSc levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrPC levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrPSc levels in N2aC24L1-3 cells, but not PrPC levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound.
International Journal of Molecular Sciences
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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ijms_22_21_11742.pdf 2.77 MB
Institute of Advanced Medical Sciences