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ID 113627
Title Alternative
マウス脳および末梢組織における年齢および性別依存的D-アミノ酸酸化酵素活性 : 加齢および神経変性への影響について
DAO activity exhibits age- and gender-dependence
Author
Kim, Soo Hyeon Tokushima University
Sogabe, Hirofumi Tokushima University
Rachadech, Wanitcha Tokushima University
Keywords
age- and gender-dependence
D-amino acid oxidase
epididymis
kidney
spinal cord
Content Type
Thesis or Dissertation
Description
D-amino acid oxidase (DAO) is a flavoenzyme, catalyzing oxidative deamination of D-amino acids to produce corresponding α-keto acids, ammonia and hydrogen peroxide. In our search for DAO activity among various tissues, we developed a sensitive assay based on hydrogen peroxide production involving enzyme-coupled colorimetric assay with peroxidase. We first optimized buffer components to extract DAO protein from mouse tissues. Here we show that DAO activity was detected in kidney, cerebellum, medulla oblongata, midbrain and spinal cord, but not in liver. In addition, we observed that DAO activity and expression were decreased in thoracic and lumbar regions of spinal cord in aged mice when compared to young mice, indicating that decreased DAO is involved in motoneuron degeneration during senescence. We also found gender difference in DAO activity in the kidney, suggesting that DAO activity is influenced by sexual dimorphism. We newly detected DAO activity in the epididymis, although undetected in testis. Furthermore, DAO activity was significantly higher in the caput region than corpus and cauda regions of epididymis, indicating that D-amino acids present in the testis are eliminated in epididymis. Taken together, age- and gender-dependent DAO activity in each organ may underlie the human pathophysiology regulated by D-amino acid metabolism.
Journal Title
The Journal of Biochemistry
ISSN
0021924X
17562651
NCID
AA12096002
AA00694073
Publisher
The Japanese Biochemical Society
Volume
166
Issue
2
Start Page
187
End Page
196
Published Date
2019-04-02
Remark
内容要旨・審査要旨・論文要約の公開
本論文は,著者Soo Hyeon Kimの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
jpn
TextVersion
その他
MEXT report number
甲第3316号
Diploma Number
甲医第1420号
Granted Date
2019-05-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Institute of Advanced Medical Sciences