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ID 110031
Title Alternative
メチオニン過剰摂取により誘導された高ホモシステイン血症は卵巣摘出ラットにおける脳動脈瘤破裂を促進する
Author
Korai, Masaaki Tokushima University|University of California
Kitazato, Keiko T. Tokushima University
Matsushita, Nobuhisa Tokushima University
Hashimoto, Tomoki Tokushima University
Keywords
Cerebral aneurysm
Hyperhomocysteinemia
Methionine
Matrix metalloproteinase-9
Folic acid
Cerebral aneurysm rupture
Anterior communicating artery
Macrophage
Tissue inhibitor of metalloproteinase-2
Content Type
Thesis or Dissertation
Description
Background: Hyperhomocysteinemia (HHcy) is associated with inflammation and a rise in the expression of matrix metalloproteinase-9 (MMP-9) in the vascular wall. However, the role of HHcy in the growth and rupture of cerebral aneurysms remains unclear.
Methods: Thirteen-week-old female Sprague-Dawley rats were subject to bilateral ovariectomy and ligation of the right common carotid artery and fed an 8 % high-salt diet to induce cerebral aneurysms. Two weeks later, they underwent ligation of the bilateral posterior renal arteries. They were divided into two groups and methionine (MET) was or was not added to their drinking water. In another set of experiments, the role of folic acid (FA) against cerebral aneurysms was assessed.
Results: During a 12-week observation period, subarachnoid hemorrhage due to aneurysm rupture was observed at the anterior communicating artery (AcomA) or the posterior half of the circle of Willis. HHcy induced by excessive MET intake significantly increased the incidence of ruptured aneurysms at 6–8 weeks. At the AcomA of rats treated with MET, we observed the promotion of aneurysmal growth and infiltration by M1 macrophages. Furthermore, the mRNA level of MMP-9, the ratio of MMP-9 to the tissue inhibitor of metalloproteinase-2, and the level of interleukin-6 were higher in these rats. Treatment with FA abolished the effect of MET, suggesting that the inflammatory response and vascular degradation at the AcomA is attributable to HHcy due to excessive MET intake.
Conclusions: We first demonstrate that in hypertensive ovariectomized rats, HHcy induced by excessive MET intake may be associated with the propensity of the aneurysm wall to rupture.
Journal Title
Journal of Neuroinflammation
ISSN
17422094
NCID
AA12051048
Publisher
Springer nature
Volume
13
Start Page
165
Published Date
2016-06-27
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704111001.pdf
論文本文:LID201704111002.pdf
本論文は, 著者Masaaki Koraiの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
Copyright: © 2016 Korai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3006号
Diploma Number
甲医第1310号
Granted Date
2017-01-26
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences