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ID 113985
Title Alternative
免疫組織化学法を用いた一側内耳破壊ラットの前庭代償の新しい評価法の開発
Author
Kitahara, Tadashi Nara Medical University
Ito, Taeko Nara Medical University
Fukushima, Munehisa Kansai Rosai Hospital
Abe, Koji Tokushima University KAKEN Search Researchers
Uno, Atsuhiko Osaka General Medical Center
Sakata-Haga, Hiromi Kanazawa Medical University KAKEN Search Researchers
Keywords
Vestibular compensation
medial vestibular nucleus
spontaneous nystagmus
Fos
thioperamide
N-methyl-D-aspartate receptor
Content Type
Thesis or Dissertation
Description
Background: Unilateral labyrinthectomy (UL) causes the disappearance of ipsilateral medial vestibular nuclear (ipsi-MVe) activity and induces spontaneous nystagmus (SN), which disappears during the initial process of vestibular compensation (VC). Ipsi-MVe-activity restores in the late process of VC.
Objective: We evaluated the late process of VC after UL in rats and examined the effects of thioperamide (H3 antagonist) on VC.
Materials and methods: MK801 (NMDA antagonist)-induced Fos-like immunoreactive (-LIR) neurons in contra-MVe, which had been suppressed by NMDA-mediated cerebellar inhibition in UL-rats was used as an index.
Results: The number of MK801-induced Fos-LIR neurons in contra-MVe gradually decreased to the same level as that of sham-operated rats 14 days after UL. Thioperamide moved the disappearance of the MK801-induced Fos-LIR neurons 2 days earlier. The number of MK801-induced Fos-LIR neurons in thioperamide-treated rats was significantly decreased, compared with that of vehicle-rats on days 7 and 12 after UL. But, thioperamide did not influence the decline of SN frequency in UL-rats.
Conclusion: There findings suggested that the number of MK801-induced Fos-LIR neurons in contra-MVe was decreased in concordance with the restoration of ipsi-MVe-activity during the late process of VC after UL and that thioperamide accelerated the late, but not the initial process of VC.
Journal Title
Acta Oto-Laryngologica
ISSN
00016489
16512251
NCID
AA0050923X
Publisher
Taylor & Francis
Volume
139
Issue
6
Start Page
505
End Page
510
Published Date
2019-04-16
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kazunori Matsudaの学位論文として提出され,学位審査・授与の対象となっている。
This is an Accepted Manuscript of an article published by Taylor & Francis in Acta Oto-Laryngologica on 16/04/2019, available online: http://www.tandfonline.com/10.1080/00016489.2019.1599140
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3339号
Diploma Number
甲医第1432号
Granted Date
2019-10-24
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences