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ID 111063
Title Alternative
ロイテリン関連化合物の歯周病原因菌への抗菌および抗バイオフィルム効果
Author
Toguchi, Munehisa Tokushima University
Keywords
Lactobacillus reuteri
reuterin
Fusobacterium nucleatum
biofilm
Content Type
Thesis or Dissertation
Description
OBJECTIVE: Lactobacillus reuteri is one of the probiotics that possesses preventive effects on oral infections including dental caries and periodontal disease. Reuterin is a bacteriocin-like compound produced by L. reuteri and plays an important role in the probiotic effects. However, it is difficult to obtain reuterin due to the general lability of its aldehyde moiety. Therefore, fourteen stable reuterin-related compounds (RRCs) were chemically synthesized. Here, we investigated the effects of RRCs on periodontopathic bacteria.
MATERIALS AND METHODS: The antibacterial activity of RRCs on pathogenic microorganisms including periodontopathic bacteria was determined. Moreover, the effects of RRCs on biofilm formation by Fusobacterium nucleatum were examined by a crystal violet biofilm formation assay. Cytotoxicity of RRCs was evaluated by a lactate dehydrogenase assay using oral keratinocytes.
RESULTS: Among synthesized RRCs, RRC-04, -05, -09, -10, -12, -13, and -14, exhibited antibacterial activities against periodontopathic bacteria. RRCs, except for RRC-06, -07, -08, and -11, significantly suppressed biofilm formation to 60-80 % of the control. Most RRCs, except for RRC-12 and -13, were not cytotoxic to human oral keratinocytes.
CONCLUSIONS: The synthesized RRCs can be potent novel oral care products for the prevention of periodontitis without adverse effects.
Journal Title
Journal of Oral Health and Biosciences
ISSN
21896682
Publisher
四国歯学会
Volume
30
Issue
1
Start Page
8
End Page
17
Sort Key
8
Published Date
2017-06-30
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704261002.pdf
審査要旨:LID201704261003.pdf
論文本文:k3076_fulltext.pdf
本論文は,著者Natsumi Fujiwaraの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3076号
Diploma Number
甲口第419号
Granted Date
2017-03-09
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
Pharmaceutical Sciences
University Hospital