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ID 115728
Author
Yamamura, Shohei National Institute of Advanced Industrial Science and Technology
Yatsushiro, Shouki National Institute of Advanced Industrial Science and Technology
Yamaguchi, Yuka National Institute of Advanced Industrial Science and Technology
Abe, Kaori National Institute of Advanced Industrial Science and Technology KAKEN Search Researchers
Kataoka, Masatoshi National Institute of Advanced Industrial Science and Technology
Keywords
microRNA
RNase protection assay
microchip electrophoresis
Content Type
Journal Article
Description
The analysis of a microRNA (miRNA), miR-222 isolated from the PC12 cell line, was performed by use of the ribonuclease (RNase) protection assay, cyanine 5 (Cy5)-labeled miR-222 riboprobe, and a Hitachi SV1210 microchip electrophoresis system, which can be used to evaluate the integrity of total RNA. The fluorescence intensity corresponding to the protected RNA fragment increased in a dose-dependent manner with respect to the complementary-strand RNA. More highly sensitive detection of miRNA by microchip electrophoresis than by conventional method using fluorescence-labeled riboprobe could be obtained in 180 s. An obvious increase in miR-222 expression induced by nerve growth factor in PC12 cells could be observed. These results clearly indicate the potential of microchip electrophoresis for the analysis of miRNA using RNase protection assay with a fluorescence-labeled riboprobe.
Journal Title
Sensors
ISSN
14248220
Publisher
MDPI
Volume
12
Issue
6
Start Page
7576
End Page
7586
Published Date
2012-05-31
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
University Hospital
Institute of Advanced Medical Sciences