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ID 115575
Title Alternative
ACTIVATION OF PORCINE OOCYTES BY CALCIUM-EDTA
Author
Morita, Yasuhiro Yamaguchi University
Taniguchi, Masayasu Yamaguchi University
Ito, Aya Yamaguchi University
Namula, Zhao Yamaguchi University
Do, Lanh Thi Kim Yamaguchi University
Takagi, Mitsuhiro Yamaguchi University
Keywords
blastocyst
Ca-EDTA
nuclear status
parthenogenesis
porcine oocyte
Content Type
Journal Article
Description
The changes triggered by sperm-induced activation of oocytes, which are required for normal oocyte development, can be mediated by other agents, thereby inducing the parthenogenesis. In this study, we exposed porcine oocytes to 1 mM Ca-EDTA, a metal-ion chelator, at various intervals during 48 hr of in vitro maturation to determine the optimum period of Ca-EDTA treatment for parthenogenetic activation. When the oocytes were cultured with or without Ca-EDTA from 36 hr (post-12), 24 hr (post-24), 12 hr (post-36) and 0 hr (post-48) after the start of maturation culture, the blastocyst formation rates were significantly higher (P<0.05) in the post-24, post-36 and post-48 groups (3.3%, 4.0% and 2.6%, respectively) than those in the control group without treatment (0%). Furthermore, when the oocytes were cultured with Ca-EDTA for 0 hr (control), 12 hr (pre-12), 24 hr (pre-24), 36 hr (pre-36) and 48 hr (pre-48) from the start of maturation culture, the oocytes formed blastocysts only in the pre-36 and pre-48 groups (0.4% or 0.8%, respectively). Pronuclei (<66.7%) were observed only when the periods of Ca-EDTA treatment were more than 12 hr during maturation culture. In the control group, no pronuclei were detected. Our findings demonstrate that porcine immature oocytes can be parthenogenetically activated by Ca-EDTA treatment for at least 24 hr to 36 hr during maturation culture, leading to pronucleus formation followed by the formation of blastocysts.
Journal Title
Journal of Veterinary Medical Science
ISSN
13477439
09167250
NCID
AA10796138
Publisher
The Japanese Society of Veterinary Science
Volume
78
Issue
6
Start Page
1019
End Page
1023
Published Date
2016-03-04
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry
Institute of Advanced Medical Sciences