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ID 118290
Title Alternative
Vibrio vulnificus創傷感染モデルマウスにおける骨格筋内の特徴的な代謝変化
Metabolic Changing in V. vulnificus-Infected Tissue
Author
Kanda, Yuna Tokushima University
Nguyen, Anh Quoc National Institute of Nutrition
Masuda, Rumiko Tokushima University
Yamazaki, Kohei Kitasato University
Kashimoto, Takashige Kitasato University
Keywords
Vibrio vulnificus
CE-TOFMS
host metabolomics
TCA cycle
MARTX toxin
Content Type
Thesis or Dissertation
Description
Vibrio vulnificus is a bacterium that inhabits warm seawater or brackish water environments and causes foodborne diseases and wound infections. In severe cases, V. vulnificus invades the skeletal muscle tissue, where bacterial proliferation leads to septicemia and necrotizing fasciitis with high mortality. Despite this characteristic, information on metabolic changes in tissue infected with V. vulnificus is not available. Here, we elucidated the metabolic changes in V. vulnificus-infected mouse skeletal muscle using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Metabolome analysis revealed changes in muscle catabolites and energy metabolites during V. vulnificus infection. In particular, succinic acid accumulated but fumaric acid decreased in the infected muscle. However, the virulence factor deletion mutant revealed that changes in metabolites and bacterial proliferation were abolished in skeletal muscle infected with a multifunctional-autoprocessing repeats-in-toxin (MARTX) mutant. On the other hand, mice that were immunosuppressed via cyclophosphamide (CPA) treatment exhibited a similar level of bacterial counts and metabolites between the wild type and MARTX mutant. Therefore, our data indicate that V. vulnificus induces metabolic changes in mouse skeletal muscle and proliferates by using the MARTX toxin to evade the host immune system. This study indicates a new correlation between V. vulnificus infections and metabolic changes that lead to severe reactions or damage to host skeletal muscle.
Journal Title
mSystems
ISSN
23795077
Publisher
American Society for Microbiology
Volume
8
Issue
2
Start Page
e00682-22
Published Date
2023-03-20
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kai Ishidaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2023 Ishida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. (https://creativecommons.org/licenses/by/4.0/)
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3734号
Diploma Number
甲栄第299号
Granted Date
2023-03-24
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences