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ID 116345
Title Alternative
細胞診における探針エレクトロスプレーイオン化質量分析の有用性 : 肺癌培養細胞での検討
PESI-MS for Diagnostic Cytology
Author
Morimoto, Yuki Tokushima University
Sumida, Satoshi Tokushima University
Kakimoto, Takumi Tokushima University
Cheng, Chunmei Novo Nordisk Research Centre China
Keywords
Mass spectrometry
Rapid cytological diagnosis
Probe electrospray ionization mass spectrometry
Lung cytopathology
Content Type
Thesis or Dissertation
Description
Objectives: Cytology and histology are 2 indispensable diagnostic tools for cancer diagnosis, which are rapidly increasing in importance with aging populations. We applied mass spectrometry (MS) as a rapid approach for swiftly acquiring nonmorphological information of interested cells. Conventional MS, which primarily rely on promoting ionization by pre-applying a matrix to cells, has the drawback of time-consuming both on data acquisition and analysis. As an emerging method, probe electrospray ionization-MS (PESI-MS) with a dedicated probe is capable to pierce sample and measure specimen in small amounts, either liquid or solid, without the requirement for sample pretreatment. Furthermore, PESI-MS is timesaving compared to the conventional MS. Herein, we investigated the capability of PESI-MS to characterize the cell types derived from the respiratory tract of human tissues. Study Design: PESI-MS analyses with DPiMS-2020 were performed on various type of cultured cells including 5 lung squamous cell carcinomas, 5 lung adenocarcinomas, 5 small-cell carcinomas, 4 malignant mesotheliomas, and 2 normal controls. Results: Several characteristic peaks were detected at around m/z 200 and 800 that were common in all samples. As expected, partial least squares-discriminant analysis of PESI-MS data distinguished the cancer cell types from normal control cells. Moreover, distinct clusters divided squamous cell carcinoma from adenocarcinoma. Conclusion: PESI-MS presented a promising potential as a novel diagnostic modality for swiftly acquiring specific cytological information.
Journal Title
Acta Cytologica
ISSN
00015547
19382650
NCID
AA00044384
AA12780809
Publisher
Karger
Volume
65
Issue
5
Start Page
430
End Page
439
Published Date
2021-06-07
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Yuki Morimotoの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2021 S. Karger AG, Basel
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3535号
Diploma Number
甲医第1502号
Granted Date
2021-09-09
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences