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ID 117783
Title Alternative
An Improved DDS Tool with Versatile Cell-targeting Ability
Author
Ohkubo, Yukimasa University of Tokushima
Tamura, Masato University of Tokushima
Ohkura, Kazuto Suzuka University
Keywords
Cholesterol-dependent cytolysin
drug delivery system
Z-domain
chimera protein
Content Type
Journal Article
Description
Background/Aim: The aim of this study was to develop an improved drug delivery system (DDS) tool with enhanced versatility in the cell-targeting step using as Z-domain, a modified IgG binding domain of protein A from Staphylococcus aureus, as an IgG adapter domain. Materials and Methods: The chimera protein expression system composed of the Z-domain and chimeric cholesterol-dependent cytolysin mutant named His-Z-CDC(ss)IS was constructed in Escherichia coli. His-Z-CDC(ss)IS was purified by Ni-affinity chromatography, and its abilities for controlled pore formation, membrane binding, IgG binding, and target cell-specific delivery of liposomes carrying medicine were investigated. Results and Discussion: His-Z-CDC(ss)IS purified by Ni-affinity chromatography indicated pore-forming activity only under disulfide bond reducing conditions. His-Z-CDC(ss)IS also demonstrated an ability to bind both IgG and cholesterol-embedded liposomes via its Z-domain and domain 4, respectively. Furthermore, anticarcinoembryonic antigen (CEA) IgG-bound His-Z-CDC(ss)IS indicated effective delivery of liposomes carrying drugs to CEA-expressing cells. Conclusion: His-Z-CDC(ss)IS was revealed to be an improved DDS tool with enhanced versatility in cell targeting.
Journal Title
Anticancer Research
ISSN
02507005
17917530
NCID
AA10625860
AA12440673
Publisher
The International Institute of Anticancer Research
Volume
33
Issue
7
Start Page
2905
End Page
2910
Published Date
2013-06-18
EDB ID
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry