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ID 117712
Title Alternative
新規PCOSラットモデルの作製とその生殖・代謝・行動表現型の評価
Author
Kamada, Shuhei Tokushima University
Aoki, Hidenori Tokushima University
Tamura, Kou Tokushima University
Takeda, Asuka Tokushima University
Masaki, Rie Tokushima University
Yanagihara, Rie Tokushima University
Hayashi, Noriko Tokushima University
Yano, Yuya Tokushima University
Imaizumi, Junki Tokushima University
Keywords
activity
DHT
metabolic
PCOS
reproduction
Content Type
Thesis or Dissertation
Description
Background: Although animal models of PCOS have been used in many studies, none of them can reproduce both the reproductive and metabolic phenotypes of PCOS. In addition, behavioral parameters have not been evaluated in PCOS animal models.
Purpose: We tried to produce an improved rat model of PCOS, and the reproductive, metabolic, and behavioral phenotypes of the model rats were evaluated.
Methods: Female rats were implanted with silicon tubes containing oil-dissolved dihydrotestosterone (Oil-DHT) as a new PCOS model. Their phenotypes were compared with those of conventional PCOS model rats (DHT), into which tubes containing crystalline DHT were implanted, and non-DHT-treated rats (control).
Results: Both the Oil-DHT and DHT rats showed greater body weight gain, food intake, and fat depot weight than the control rats. Furthermore, these groups showed fewer estrous stages and increased numbers of cystic follicles. The DHT rats exhibited lower ovarian and uterine weights than the control rats, whereas no such changes were observed in the Oil-DHT rats. The Oil-DHT and DHT rats showed less locomotor activity in the light phase than the control rats.
Conclusions: Our proposed PCOS model reproduced both the reproductive and metabolic phenotypes of PCOS and may have potential for PCOS research.
Journal Title
Reproductive Medicine and Biology
ISSN
14470578
NCID
AA11706516
Publisher
Japan Society for Reproductive Medicine|John Wiley & Sons
Volume
21
Issue
1
Start Page
e12416
Published Date
2021-10-02
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shuhei Kamadaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3796号
Diploma Number
甲医第1602号
Granted Date
2024-03-22
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences