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ID 117616
Title Alternative
Porphyromonas gingivalisの外膜小胞は歯肉上皮細胞を刺激しMAPKとSTING経路を介して炎症性サイトカインを産生させる
Author
Tada, Ayano Kagawa University
Kuwahara, Tomomi Kagawa University
Keywords
Porphyromonas gingivalis
outer membrane vesicles
stimulator of interferon genes
inflammation
periodontitis
Content Type
Thesis or Dissertation
Description
Porphyromonas gingivalis (Pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. Pg-OMVs are involved in the pathogenesis of periodontitis. Pg-OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of Pg-OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. Pg-OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. Pg-OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that Pg-OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that Pg-OMVs may play important roles in periodontitis exacerbation by stimulating various pathways.
Journal Title
Biomedicines
ISSN
22279059
Publisher
MDPI
Volume
10
Issue
10
Start Page
2643
Published Date
2022-10-20
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Yuta Uemuraの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3712号
Diploma Number
甲口第491号
Granted Date
2023-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
University Hospital
Institute of Advanced Medical Sciences
Oral Sciences