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ID 115319
Title Alternative
腸炎ビブリオにおけるトランスポゾン変異株を用いた長波長紫外線(UVA)感受性に関連する遺伝子の同定
Author
Maetani-Yasui, Miki Tokushima University
Honjo, Airi Tokushima University
Bui, Thi Kim Ngan Tokushima University
Keywords
Vibrio parahaemolyticus
ultraviolet
light-emitting diodes
UV-LED
Oxidative stress
light-emitting diode
ultraviolet A
Content Type
Thesis or Dissertation
Description
Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.
Journal Title
Applied Sciences
ISSN
20763417
Publisher
MDPI
Volume
10
Issue
16
Start Page
5549
Published Date
2020-08-11
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Miki Maetani-Yasuiの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3462号
Diploma Number
甲栄第281号
Granted Date
2020-09-24
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
Science and Technology