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ID 112928
Title Alternative
ナローバンドUVBがHeLa細胞とアレルギー性鼻炎モデルラットの鼻粘膜におけるヒスタミンH1受容体遺伝子発現の亢進とアポトーシスの誘導に与える影響
Author
Okamoto, Kentaro Tokushima University
Sanada, Nanae Tokushima University
Yamada, Takuya Tokushima University
Sugiyama, Manabu Tokushima University
Michinaga, Shotaro Osaka Ohtani University
Kitayama, Mika Tokushima University
Keywords
Allergic rhinitis
apoptosis
histamine H1 receptor gene
narrowband-ultraviolet B
phototherapy
Content Type
Thesis or Dissertation
Description
Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12- myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and upregulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR.
Journal Title
Journal of Pharmacological Sciences
ISSN
13478613
NCID
AA12022662
Publisher
Elsevier|Japanese Pharmacological Society
Volume
138
Issue
1
Start Page
54
End Page
62
Published Date
2018-09-10
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Tatsuya Fujiiの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3246号
Diploma Number
甲医第1395号
Granted Date
2018-11-22
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences
Pharmaceutical Sciences