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ID 115332
Title Alternative
マウスにおける動脈硬化性急性大動脈症候群の発症に対するケルセチンの予防効果
Author
Kondo, Masateru Tokushima University
Hosooka, Mayuko Tokushima University
Kagimoto, Yuu Tokushima University
Saito, Naoko Tokushima University
Matsuoka, Rie Tokushima University
Chuma, Masayuki Tokushima University
Keywords
quercetin
aortic aneurysm
aortic dissection
endothelial dysfunction
flavonoids
inflammation
Content Type
Thesis or Dissertation
Description
Atherosclerosis-related acute aortic syndromes, such as aortic aneurysms or aortic dissection are life-threatening diseases. Since they develop suddenly and progress rapidly, the establishment of preventive strategies is urgently needed. Quercetin, a flavonoid abundant in various vegetables and fruits, is suggested to reduce the risk of cardiovascular disease. Therefore, in this study, the preventive effect of quercetin was evaluated using a mouse model of aortic aneurysm and dissection. The model was established by administering angiotensin II (Ang II) and β-aminopropionitrile (BAPN), a lysyl oxidase inhibitor, to mice to induce hypertension and degeneration of the elastic lamina, which would eventually result in the onset of an aortic aneurysm. Ang II, BAPN, and a nitric oxide synthase inhibitor was administered to induce aortic dissection via endothelial dysfunction. Quercetin (60 mg/kg/day) was administered 2 weeks before inducing aortic diseases by the end of the experiments (8 weeks in the aneurysm model, 6 weeks in the dissection model). It was found to reduce the incidence of aneurysm (from 72 to 45%), dissection (from 17 to 10%), and rupture (from 33 to 15%) in mice. Elastin degradation was ameliorated in the quercetin-treated mice compared to that in the mice without quercetin treatment (degradation score 2.9 ± 0.3 vs 2.2 ± 0.2). Furthermore, quercetin suppressed the expression of vascular cell adhesion molecule-1, macrophage infiltration, and pro-matrix metalloproteinase-9 activity. Our results suggest that quercetin might prevent the onset of atherosclerosis-related acute aortic syndromes through its anti-inflammatory and endothelial cell-protective effects.
Journal Title
International Journal of Molecular Sciences
ISSN
14220067
16616596
Publisher
MDPI
Volume
21
Issue
19
Start Page
7226
Published Date
2020-09-30
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Masateru Kondoの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3501号
Diploma Number
甲医第1487号
Granted Date
2021-03-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital