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ID 117908
Author
Husseini, Rabab A. Zagazig University|Tokushima University
Hasan, Azza A. Zagazig University
El Megrab, Nagia A. Zagazig University
Keywords
prophylactic cancer vaccine
iontophoresis
polyplex nanoparticle
antigen peptide
cytosine-phosphate- guanosine motif (CpG-ODN) adjuvant
melanoma
Content Type
Journal Article
Description
Although the strategy in cancer vaccination is to provide a therapeutic effect against an established tumor, there is an urgent need to develop prophylactic vaccines for non-viral cancers. In this study, we prepared polyplex nanoparticles through electrostatic interactions between a positively-charged modified tumor associated antigen, namely human derived melanoma gp10025–33 peptide (KVPRNQDWL-RRRR), and a negatively charged cytosine-phosphate-guanosine motif (CpG-ODN) adjuvant. We previously demonstrated successful transdermal delivery of various hydrophilic macromolecules by iontophoresis (IP) using weak electricity. Herein, we investigated the effectiveness of IP in the transdermal delivery of a prophylactic polyplex vaccine. IP was successful in establishing a homogenous distribution of the vaccine throughout skin. Efficacy of the vaccine was demonstrated against melanoma growth. A significant tumor regression effect was observed, which was confirmed by elevated mRNA expression levels of various cytokines, mainly interferon (IFN)-γ, as well as infiltration of cytotoxic CD8+ T cells. Additionally, we evaluated the therapeutic effect of the vaccine and we found a significant reduction in tumor burden. Stimulation of systemic immunity was confirmed by upregulation of IFN-γ. This is the first report to demonstrate the use of IP in the transdermal delivery of a prophylactic melanoma vaccine.
Journal Title
Biological and Pharmaceutical Bulletin
ISSN
13475215
NCID
AA11696048
Publisher
The Pharmaceutical Society of Japan
Volume
46
Issue
3
Start Page
494
End Page
504
Published Date
2023-03-01
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences