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ID 106279
Title Alternative
Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響
Effect of eDNA on biofilm formation
Author
Asikin, Nur The University of Tokushima
Hirota, Katsuhiko The University of Tokushima KAKEN Search Researchers
Hirao, Kouji The University of Tokushima KAKEN Search Researchers
Liu, Dali Shanghai Jiao Tong University
Takahashi, Kanako The University of Tokushima KAKEN Search Researchers
Shu, Rong Shanghai Jiao Tong University
Keywords
extracellular DNA
extracellular HLP
biofilm development
Streptococcus intermedius
DNA concentration
Content Type
Thesis or Dissertation
Description
Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.
Methods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains.
Conclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity.
Significance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
Journal Title
Journal of Applied Microbiology
ISSN
13652672
13645072
NCID
AA11115564
Publisher
The Society for Applied Microbiology|John Wiley & Sons Inc
Volume
115
Issue
1
Start Page
260
End Page
270
Published Date
2013-03-29
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201409191007.pdf
審査要旨 : LID201409191008.pdf
論文本文 : k2757_fulltext.pdf
本論文は,著者Asikin Nurの学位論文として提出され,学位審査・授与の対象となっている。
著者の申請により要約(2014-09-20公開)に替えて論文全文を公開(2019-10-09)
Rights
This is the peer reviewed version of the following article: Nur, A., Hirota, K., Yumoto, H., Hirao, K., Liu, D., Takahashi, K., Murakami, K., Matsuo, T., Shu, R. and Miyake, Y. (2013), Effects of extracellular DNA and DNA‐binding protein on the development of a Streptococcus intermedius biofilm. J Appl Microbiol, 115: 260-270., which has been published in final form at https://doi.org/10.1111/jam.12202. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2757号
Diploma Number
甲口第386号
Granted Date
2014-09-10
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital