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ID 110103
Title Alternative
genipinはTNF-αが誘導するヒト歯根膜細胞のMMP-1およびMMP-3産生を抑制する
Author
Shindo, Satoru The University of Tokushima
Keywords
Genipin
MMP
AMPK
MAPKs
Periodontal ligament cells
Content Type
Thesis or Dissertation
Description
Genipin, the aglycon of geniposide found in gardenia fruit has long been considered for treatment of inflammatory diseases in traditional oriental medicine. Genipin has recently been reported to have some pharmacological functions, such as antimicrobial, antitumor, and anti-inflammatory effects. The aim of this study was to examine whether genipin could modify matrix metalloproteinase (MMP)-1 and MMP-3, which are related to the destruction of periodontal tissues in periodontal lesion, expression in tumor necrosis factor (TNF)-a-stimulated human periodontal ligament cells (HPDLCs). Genipin prevented TNF-a- mediated MMP-1 and MMP-3 productions in HPDLCs. Moreover, genipin could suppress not only extracellular signal-regulated kinase (ERK) and Jun-N-terminal kinase (JNK) phosphorylations but also AMP-activated protein kinase (AMPK) phosphorylation in TNF-a-stimulated HPDLCs. Inhibitors of ERK and AMPK could inhibit both MMP-1 and MMP-3 productions. Moreover, we revealed the ERK inhibitor suppressed AMPK phosphorylation in TNF-a-stimulated HPDLCs. These data provide a new mechanism through which genipin could be used for the treatment of periodontal disease to prevent MMPs expression in periodontal lesion.
Journal Title
Biochimie
ISSN
03009084
NCID
AA11522535
AA00564792
Publisher
Elsevier B.V.|Société Française de Biochimie et Biologie Moléculaire (SFBBM).
Volume
107
Issue
B
Start Page
391
End Page
395
Published Date
2014-10-18
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704261014.pdf
審査要旨:LID201704261015.pdf
論文本文:LID201704261016.pdf
本論文は, 著者Satoru Shindoの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3082号
Diploma Number
甲口第425号
Granted Date
2017-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences