ID | 116724 |
Author |
Tadokoro, Susumu
Tsurumi University
Tokuyama-Toda, Reiko
Tsurumi University
Tatehara, Seiko
Tsurumi University
Ide, Shinji
Tsurumi University
Umeki, Hirochika
Tsurumi University
Miyoshi, Keiko
Tokushima University
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Noma, Takafumi
Hiroshima Jogakuin University
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Satomura, Kazuhito
Tsurumi University
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Keywords | iPSC
differentiation
frozen section
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Content Type |
Journal Article
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Description | Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liver, the brain, the spinal cord, and cover glasses (control) for 9 days. The iPSCs cultured on the sections of the liver resembled hepatocytes, whereas those on sections of the brain and the spinal cord resembled neuronal cells. The percentage of hepatocytic marker-positive cells in the iPSCs cultured on the sections of the liver was statistically higher than that of those in the iPSCs cultured on the sections of the brain and the spinal cord or on cover glasses. In contrast, the iPSCs cultured on the sections of the brain and the spinal cord revealed a high percentage of neural marker-positive cells. Thus, iPSCs can be differentiated into a specific cell lineage in response to specific factors within frozen sections of tissues/organs. Differentiation efficacy of the frozen sections markedly differed between the iPSC clones. Therefore, our induction method could be simple and effective for evaluating the iPSC quality.
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Journal Title |
Cells
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ISSN | 20734409
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Publisher | MDPI
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Volume | 10
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Issue | 11
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Start Page | 2827
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Published Date | 2021-10-21
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Rights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
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language |
eng
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TextVersion |
Publisher
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departments |
Oral Sciences
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