ID | 111642 |
Author |
Ogushi, Fumitaka
The University of Tokushima
Takahashi, Hiroko
The University of Tokushima
Kawano, Tetsuya
The University of Tokushima
Endo, Takeshi
The University of Tokushima
Sone, Saburo
The University of Tokushima
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Keywords | thrombin
fibroblast growth-stimulating factor
platelet-derived growth factor
pulmonary fibrosis
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Content Type |
Journal Article
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Description | Thrombin is a multifunctional enzyme generated at sites of vascular injury, and is known to be increased in the lungs in some types of fibrotic lung disease. In this study, to determine whether thrombin is associated with fibroblast growth and pulmonary fibrosis in these disorders, we examined whether a growth factor for fibroblasts (platelet-derived growth factor, PDGF) was released by thrombin-stimulated alveolar macrophages (AM). The culture supernatants of rat AM stimulated with 1 or 10 U/ml of thrombin showed a significant increase in fibroblast growth-stimulating activity (FGA). Pretreatment of the AM supernatant with anti-PDGF-AA antibody significantly decreased the FGA, but pretreatment with anti-PDGF-BB antibody did not. The supernatants of AM stimulated with thrombin also increased the growth of fibroblasts from the lungs of rats with bleomycin-induced lung injury. These results indicate that thrombin stimulates AM to release PDGF-AA, which is responsible, at least in part, for fibroblast growth and the development of pulmonary fibrosis in some types of fibrotic lung disease.
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Journal Title |
The Journal of Medical Investigation
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ISSN | 13431420
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NCID | AA11166929
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Publisher | The University of Tokushima School of Medicine
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Volume | 44
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Issue | 1-2
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Start Page | 59
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End Page | 65
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Sort Key | 59
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Published Date | 1997-08
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URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
Publisher
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departments |
University Hospital
Medical Sciences
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