Total for the last 12 months
number of access : ?
number of downloads : ?
ID 115722
Author
Jabasini, Mohammad The University of Tokushima|Nagoya University
Ewis, Ashraf The University of Tokushima
Nakahori, Yutaka The University of Tokushima
Baba, Yoshinobu Nagoya University|National Institute of Advanced Industrial Science and Technology
Keywords
anomalous separation
DNA
microchip electrophoresis
Content Type
Journal Article
Description
We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.
Journal Title
Scientia Pharmaceutica
ISSN
22180532
Publisher
MDPI
Volume
84
Issue
3
Start Page
507
End Page
513
Published Date
2016-05-26
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences