ID | 115825 |
Title Alternative | Molecular analysis for bacterial contamination
|
Author |
Watanabe, Akari
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Tamaki, Naofumi
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Matsuyama, Miwa
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Kokeguchi, Susumu
Okayama University
|
Keywords | Bacterial contamination
Dental unit water line
Molecular analysis
PCR-DGGE
|
Content Type |
Journal Article
|
Description | Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.
|
Journal Title |
New Microbiologica
|
ISSN | 11217138
|
NCID | AA11980981
|
Publisher | EDIMES
|
Volume | 39
|
Issue | 2
|
Start Page | 143
|
End Page | 145
|
Published Date | 2016-04
|
EDB ID | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
Publisher
|
departments |
Oral Sciences
|