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ID 110137
Title Alternative
ヒト腱・靭帯のプロテオーム解析 : 結合組織における不溶性細胞外マトリックスの可溶化と解析
Author
Goda, Yuichiro Tokushima University
Kosaka, Hirofumi Tokushima University
Keywords
不溶性細胞外マトリックス
コラーゲン
エラスチン
プロテオーム解析
yellow ligament
Achilles tendon
elastin
collagen
proteome
Content Type
Thesis or Dissertation
Description
Connective tissues such as tendon, ligament and cartilage are mostly composed of extracellular matrix (ECM). These tissues are insoluble, mainly due to the highly cross-linked ECM proteins such as collagens. Difficulties obtaining suitable samples for mass spectrometric analysis render the application of modern proteomic technologies difficult. Complete solubilization of them would not only elucidate protein composition of normal tissues but also reveal pathophysiology of pathological tissues. Here we report complete solubilization of human Achilles tendon and yellow ligament, which is achieved by chemical digestion combined with successive protease treatment including elastase. The digestion mixture was subjected to liquid chromatography-mass spectrometry. The low specificity of elastase was overcome by accurate mass analysis achieved using FT-ICR-MS. In addition to the detailed proteome of both tissues, we also quantitatively determine the major protein composition of samples, by measuring peak area of some characteristic peptides detected in tissue samples and in purified proteins. As a result, differences between human Achilles tendon and yellow ligament were elucidated at molecular level.
Journal Title
Journal of Proteome Research
ISSN
15353893
NCID
AA11638846
AA12442770
Publisher
ACS Publications
Volume
15
Issue
12
Start Page
4709
End Page
4721
Published Date
2016-10-17
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201705191001.pdf
論文本文:k3009_fulltext.pdf
著者の申請により要約(2017-05-19公開)に替えて論文全文を公開(2018-06-14)
本論文は, 著者Nori Satoの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
Copyright © 2016 American Chemical Society
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3009号
Diploma Number
甲医第1313号
Granted Date
2017-02-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Institute of Advanced Medical Sciences
Medical Sciences