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ID 114526
Title Alternative
乳癌捺印細胞診検体のHER2およびKi67免疫染色は組織診断に代替可能である
Author
Morimoto, Masami The University of Tokushima
Nakagawa, Misako The University of Tokushima KAKEN Search Researchers
Takechi, Hirokazu The University of Tokushima KAKEN Search Researchers
Yoshida, Takahiro The University of Tokushima KAKEN Search Researchers
Honda, Junko Higashi Tokushima Medical Center
Tadokoro, Yukiko The University of Tokushima KAKEN Search Researchers
Moriya, Takuya Kawasaki Medical School
Sasa, Mitsunori Tokushima Breast Care Clinic
Keywords
Breast cancer
HER2
Ki67
Immunocytochemical analysis
Touch-smear cells
Content Type
Thesis or Dissertation
Description
Background: Re-evaluation of the subtype of recurrent breast cancer is necessary for deciding the treatment approach, but it is often not performed due to the difficulty of obtaining tissue specimens from a recurrent lesion, etc. However, when a recurrent lesion is close to the body surface, fine-needle aspiration cells (FNA cells) can be easily obtained, and immunocytochemical (ICC) analysis of hormone receptors expression in FNA cells is said to be highly reliable. However, there is no consensus regarding ICC analysis of human epidermal growth factor receptor type2 (HER2) expression and the Ki67 index using FNA cells.
Methods: Touch-smear cells (TSC) were prepared from resected specimens from 36 patients with primary invasive ductal carcinoma of the breast. The TSC were fixed in 95% ethanol and subjected to ICC analysis for HER2 using HercepTestTM (Dako) and Ki67 using MIB-1TM (Dako). HER2 expression and the Ki67 index for the TSC were compared with the results of immunohistochemical analysis of histological section (HS). Statistical analyses used the kappa test and Pearson’s correlation coefficients.
Results: HER2 and Ki67 were analyzed in TSC from 36 and 28 patients, respectively. The HER2 expression scores in the TSC and HS groups showed good agreement (kappa value=0.640), and significant correlation (correlation coefficient=0.860, p<0.001). The Ki67 indexes in the TSC and HS groups also showed significant correlation (correlation coefficient=0.861, p<0.001).
Conclusions: The reliability of ICC analysis of HER2 expression and the Ki67 index using TSC were recognized.
Journal Title
Breast Cancer
ISSN
13406868
18804233
NCID
AA1103354X
Publisher
Springer Nature|The Japanese Breast Cancer Society
Volume
23
Issue
4
Start Page
577
End Page
582
Published Date
2015-03-26
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Masami Morimotoの学位論文として提出され,学位審査・授与の対象となっている。
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s12282-015-0605-z
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
乙第2112号
Diploma Number
乙医第1760号
Granted Date
2020-03-18
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital