ID | 114526 |
Title Alternative | 乳癌捺印細胞診検体のHER2およびKi67免疫染色は組織診断に代替可能である
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Author |
Morimoto, Masami
The University of Tokushima
Bando, Yoshimi
Tokushima University
Tokushima University Educator and Researcher Directory
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Honda, Junko
Higashi Tokushima Medical Center
Moriya, Takuya
Kawasaki Medical School
Sasa, Mitsunori
Tokushima Breast Care Clinic
Tangoku, Akira
The University of Tokushima
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Keywords | Breast cancer
HER2
Ki67
Immunocytochemical analysis
Touch-smear cells
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Content Type |
Thesis or Dissertation
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Description | Background: Re-evaluation of the subtype of recurrent breast cancer is necessary for deciding the treatment approach, but it is often not performed due to the difficulty of obtaining tissue specimens from a recurrent lesion, etc. However, when a recurrent lesion is close to the body surface, fine-needle aspiration cells (FNA cells) can be easily obtained, and immunocytochemical (ICC) analysis of hormone receptors expression in FNA cells is said to be highly reliable. However, there is no consensus regarding ICC analysis of human epidermal growth factor receptor type2 (HER2) expression and the Ki67 index using FNA cells.
Methods: Touch-smear cells (TSC) were prepared from resected specimens from 36 patients with primary invasive ductal carcinoma of the breast. The TSC were fixed in 95% ethanol and subjected to ICC analysis for HER2 using HercepTestTM (Dako) and Ki67 using MIB-1TM (Dako). HER2 expression and the Ki67 index for the TSC were compared with the results of immunohistochemical analysis of histological section (HS). Statistical analyses used the kappa test and Pearson’s correlation coefficients. Results: HER2 and Ki67 were analyzed in TSC from 36 and 28 patients, respectively. The HER2 expression scores in the TSC and HS groups showed good agreement (kappa value=0.640), and significant correlation (correlation coefficient=0.860, p<0.001). The Ki67 indexes in the TSC and HS groups also showed significant correlation (correlation coefficient=0.861, p<0.001). Conclusions: The reliability of ICC analysis of HER2 expression and the Ki67 index using TSC were recognized. |
Journal Title |
Breast Cancer
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ISSN | 13406868
18804233
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NCID | AA1103354X
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Publisher | Springer Nature|The Japanese Breast Cancer Society
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Volume | 23
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Issue | 4
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Start Page | 577
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End Page | 582
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Published Date | 2015-03-26
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Masami Morimotoの学位論文として提出され,学位審査・授与の対象となっている。 This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s12282-015-0605-z |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 乙第2112号
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Diploma Number | 乙医第1760号
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Granted Date | 2020-03-18
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
University Hospital
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