Actomyosin organization at adherens junctions
Sakakibara, Shotaro Kobe University|Tokushima University Tokushima University Educator and Researcher Directory
Mizutani, Kiyohito Kobe University
Sugiura, Ayumu Kobe University
Sakane, Ayuko Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sasaki, Takuya Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yonemura, Shigenobu RIKEN|Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takai, Yoshimi Kobe University
Actomyosin-undercoated adherens junctions are critical for epithelial cell integrity and remodeling. Actomyosin associates with adherens junctions through αE-catenin complexed with β-catenin and E-cadherin in vivo; however, in vitro biochemical studies in solution showed that αE-catenin complexed with β-catenin binds to F-actin less efficiently than αE-catenin that is not complexed with β-catenin. Although a “catch-bond model” partly explains this inconsistency, the mechanism for this inconsistency between the in vivo and in vitro results remains elusive. We herein demonstrate that afadin binds to αE-catenin complexed with β-catenin and enhances its F-actin–binding activity in a novel mechanism, eventually inducing the proper actomyosin organization through αE-catenin complexed with β-catenin and E-cadherin at adherens junctions.
Journal of Cell Biology
Rockefeller University Press
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