ID 110676
Author
Kano, Mihoko Department of Nutrition, The University of Tokushima School of Medicine
Kitano, Takako Department of Nutrition, The University of Tokushima School of Medicine
Ikemoto, Madoka Department of Nutrition, The University of Tokushima School of Medicine|Department of Neuromuscular Research, National Center of Neurology and Psychiatry
Hirasaka, Katsuya Department of Nutrition, The University of Tokushima School of Medicine KAKEN Search Researchers
Asanoma, Yuki Department of Nutrition, The University of Tokushima School of Medicine
Ogawa, Takayuki Department of Orthopedic Surgery, The University of Tokushima School of Medicine
Takeda, Shin’ichi Department of Neuromuscular Research, National Center of Neurology and Psychiatry
Nonaka, Ikuya National Institute of Neuroscience, National Center of Neurology and Psychiatry
Adams, Gregory R Department of Physiology and Biophysics, University of California
Baldwin, Kenneth M Department of Physiology and Biophysics, University of California
Oarada, Motoko Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University
Kishi, Kyoichi Department of Nutrition, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nikawa, Takeshi Department of Nutrition, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
spaceflight
differential display approach
skeletal muscle gene
rats
Content Type
Journal Article
Description
We obtained the skeletal muscle of rats exposed to weightless conditions during a 16-day-spaceflight (STS-90). By using a differential display technique, we identified 6 up-regulated and 3 down-regulated genes in the gastrocnemius muscle of the spaceflight rats, as compared to the ground control. The up-regulated genes included those coding Casitas B-lineage lymphoma-b, insulin growth factor binding protein-1, titin and mitochondrial gene 16 S rRNA and two novel genes (function unknown). The down-regulated genes included those encoding RNA polymerase II elongation factor-like protein, NADH dehydrogenase and one novel gene (function unknown). In the present study, we isolated and characterize done of two novel muscle genes that were remarkably up-regulated by spaceflight. The deduced amino acid sequence of the spaceflight-induced gene (sfig) comprises 86amino acid residues and is well conserved from Drosophila to Homo sapiens. A putative leucine-zipper structure located at the N-terminal region of sfig suggests that this gene may encode a transcription factor. The up-regulated expression of this gene, confirmed by Northern blot analysis, was observed not only in the muscles of spaceflight rats but also in the muscles of tail-suspended rats, especially in the early stage of tail-suspension when gastrocnemius muscle atrophy initiated. The gene was predominantly expressed in the kidney, liver, small intestine and heart. When rat myoblastic L6 cells were grown to 100% confluence in the cell culture system, the expression of sfig was detected regardless of the cell differentiation state. These results suggest that spaceflight has many genetic effects on rat skeletal muscle.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
50
Issue
1-2
Start Page
39
End Page
47
Sort Key
39
Published Date
2003
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences