ID 79136
Author
Kawano, Takashi Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Tanaka, Katsuya Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yin, hua Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Eguchi, Satoru Department of Dental Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kawano, Hiroaki Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School
Takahashi, Akira Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nakaya, Yutaka Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Oshita, Shuzo Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
intravenous anesthetics
ketamine
nicorandil
potassium channel
patch-clamp configuration
Content Type
Journal Article
Description
Purpose : Nicorandil opens adenosine triphosphate-sensitive potassium (KATP)
channels in the cardiovascular system and is being increasingly used for the treatment
of angina pectoris. In the present study, we tested whether intravenous anesthetic agent
ketamine affected nicorandil-induced native vascular KATP channel activation. Methods :
We used excised inside-out patch clamp configurations to investigate the direct effects
of ketamine racemate and S-(+)-ketamine on the activities of KATP channels in cultured
rat aortic smooth muscle cells. Furthermore, we also investigated whether intracellular
MgADP could modulate ketamine inhibition. Results : Nicorandil significantly activated
KATP channel activity, whereas this channel activity was completely blocked by glibenclamide,
a specific KATP channel blocker. Ketamine racemate inhibited the nicorandil induced
KATP channel activity (IC50=34 1M, n=14), but S-(+)-ketamine was less potent than ketamine
racemate in blocking nicorandil induced KATP channel activities (IC50=226 7M,
n=10). Application of MgADP to the intracellular side of the channel was able to decrease
the inhibitory potency of ketamine racemate on nicorandil induced KATP channel activities.
Conclusions : Our results indicate that ketamine inhibits nicorandil induced KATP channel
activities in a dose dependent and stereoselective manner. Furthermore, increase of
intracellularMgADP attenuates the inhibitory potency of ketamine racemate.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
57
Issue
3-4
Start Page
237
End Page
244
Sort Key
237
Published Date
2010-08
Remark
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
FullText File
language
eng
departments
Medical Sciences
Oral Sciences
University Hospital