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ID 111713
Title Alternative
シェーグレン症候群疾患モデルマウスにおける眼病変の病理学的解析
Author
Ushio, Aya Tokushima University
Eguchi, Hiroshi Sakai Hospital Kindai University KAKEN Search Researchers
Hotta, Fumika Sakai Hospital Kindai University
Keywords
Sjögren’s Syndrome
dry eye
lacrimal glands
cytokine
chemokine
keratoconjunctivitis
tears
Content Type
Thesis or Dissertation
Description
Sjögren’s syndrome (SS) is a systemic autoimmune disease characterized by severe inflammation of exocrine glands such as the salivary and lacrimal glands. When it affects the lacrimal glands, many patients experience keratoconjunctivitis due to severely dry eyes. This study investigated the pathological and immunological characteristics of ocular lesions in a mouse model of SS. Corneal epithelial injury and hyperplasia were confirmed pathologically. The number of conjunctival mucin-producing goblet cells was significantly decreased in the SS model mice compared with control mice. Expression levels of transforming growth factor (TGF)-β, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and C-X-C motif chemokine (CXCL) 12 were significantly higher in the corneal epithelium of the SS model mice than in control mice. Inflammatory lesions were observed in the Harderian, intraorbital, and extraorbital lacrimal glands in the SS model mice, suggesting that the ocular glands were targeted by an autoimmune response. The lacrimal glands of the SS model mice were infiltrated by cluster of differentiation (CD)4+ T cells. Real-time reverse transcription-polymerase chain reaction (RT-PCR) revealed significantly increased mRNA expression of TNF-α, TGF-β, CXCL9, and lysozyme in the extraorbital lacrimal glands of the SS model mice compared with control mice. These results add to the understanding of the complex pathogenesis of SS and may facilitate development of new therapeutic strategies.
Journal Title
International Journal of Molecular Sciences
ISSN
14220067
16616596
NCID
AA12038549
Publisher
MDPI
Volume
18
Issue
6
Start Page
1209
Published Date
2017-06-06
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Aya Ushioの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/ ).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3173号
Diploma Number
甲口第430号
Granted Date
2018-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital