ID 113372
Title Alternative
TNF-α短期刺激による骨髄由来間葉系幹細胞の細胞動態解析
Effect of Short Term TNF-α Stimulation of BMSCs
Author
Naritani, Mio Tokushima University
Raju, Resmi Tokushima University
Keywords
TNF-α
Bone marrow-derived mesenchymal stem cells
Short-term stimulation
Cell proliferation
Cell differentiation
Content Type
Thesis or Dissertation
Description
Bone marrow-derived mesenchymal stem cells (BMSCs) have considerable potential for self-renewal and multi-differentiation. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine and is involved in tissue regeneration during wound healing. It was already reported that cultured human dental pulp cells acquire stem cell properties following short-term stimulation by TNF-α. However, it has not been clarified if BMSCs acquire stem cell properties after TNF-α treatment. The purpose of this study was to investigate the effect of short-term stimulation with TNF-α on BMSCs. Rat BMSCs were cultured up to 60% confluence and then incubated with 1–100 ng/ml of recombinant rat TNF-α (rTNF-α) for a further 2 days. After reaching subconfluence, cells were passaged once to remove rTNF-α completely before subsequent assays. Cells in the control group were passaged without stimulation. Expression levels of Nanog and Oct4 stem cell markers were significantly increased in the rTNF-α 10 ng/ml stimulation group. rTNF-α stimulation did not affect cell proliferation compared with the control group. However, rTNF-α stimulation led to a delay in cell differentiation. This study suggests that short-term TNF-α stimulation of BMSCs significantly increased their stem cell phenotype, but delayed their osteogenic cell differentiation.
Journal Title
Journal of Hard Tissue Biology
ISSN
13417649
1880828X
NCID
AA11074332
Publisher
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
Volume
28
Issue
2
Start Page
99
End Page
108
Published Date
2019-04-24
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mio Naritaniの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3282号
Diploma Number
甲口第447号
Granted Date
2019-03-22
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital