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ID 109488
Title Alternative
ヒストンデメチラーゼJmjd3は転写因子Runx2とOsterixを介して骨芽細胞の分化を調節する
Regulation of osteoblast differentiation by Jmjd3
Author
Yang, Di The University of Tokushima|China Medical University
Okamura, Hirohiko The University of Tokushima KAKEN Search Researchers
Nakashima, Yoshiki The University of Tokushima KAKEN Search Researchers
Keywords
Jmjd3
H3K27me3
BMP-2
Osteoblast differentiation
Histone demethylation
Runx2
Osterix
Content Type
Thesis or Dissertation
Description
Post-translational modifications of histones including methylation play important roles in cell differentiation. Jumonji domain-containing 3 (Jmjd3) is a histone demethylase, which specifically catalyzes the removal of trimethylation of histone H3 at lysine 27 (H3K27me3). In this study, we examined the expression of Jmjd3 in osteoblasts and its roles in osteoblast differentiation. Jmjd3 expression in the nucleus was induced in response to the stimulation of osteoblast differentiation as well as treatment of bone morphogenetic protein-2 (BMP-2). Either treatment with Noggin, an inhibitor of BMP-2, or silencing of Smad1/5, suppressed Jmjd3 expression during osteoblast differentiation. Silencing of Jmjd3 expression suppressed osteoblast differentiation through the expression of bone-related genes including Runx2, Osterix, Osteopontin, Bone sialoprotein (BSP), and Osteocalcin (OCN). Silencing of Jmjd3 decreased the promoter activities of Runx2 and Osterix and increased the level of H3K27me3 on the promoter regions of Runx2 and Osterix. Introduction of the exogenous Runx2 and Osterix partly rescued osteoblast differentiation in the shJmjd3 cells. The present results indicate that Jmjd3 plays important roles in osteoblast differentiation and regulates the expressions of BSP and OCN via transcription factors Runx2 and Osterix.
Journal Title
The Journal of Biological Chemistry
Volume
288
Issue
47
Start Page
33530
End Page
33541
Published Date
2013-10-8
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201509181013.pdf
審査要旨 : LID201509181014.pdf
論文本文 : LID201509181015.pdf
本論文は, 著者Di Yangの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
Copyright : The American Society for Biochemistry and Molecular Biology.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2862号
Diploma Number
甲口第401号
Granted Date
2015-09-16
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
Bioscience and Bioindustry