| ID | 101801 |
| Title Transcription | セイブンカ ト セイシ ケイセイ キコウ ニ タイスル プロテオミクス カラノ アプローチ
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| Title Alternative | Proteomics approach for the mechanism of sex determination
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| Author |
Sato, Youichi
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
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Shinka, Toshikatsu
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Chen, Gang
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Yan, Hong-Tao
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Sakamoto, Kozue
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Yang, Xin‐Jun
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Umeno, Mayumi
School of Health Science, The University of Tokushima Faculty of Medicine
Nakahori, Yutaka
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
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| Keywords | SRY
sex determination
Y chromosome
proteomics
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| Content Type |
Journal Article
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| Description | SRY （sex determining region on the Y chromosome） was a gene isolated as the testis determining factor on the Y chromosome in １９９０．The gene consisted of single exon and encodes a ２０４ amino acid protein. Since SRY protein has a HMG domain, which functions as DNA binding domain, it has been considered to be a transcription factor. Direct target of SRY, however, have not yet identified for １４ years.
In this study, we have performed a proteomics approach to analyze the function of SRY. Twodimensional gel electrophoresis after over expression of SRY showed considerable down regulation in many proteins. Among the proteins, mitochondrial ６０‐kDa heat shock protein （HSP６０） and probable protein disulfide isomerase （ER６０） remarkably decreased. Since they were reported to be associated with cell proliferation and differentiation, we investigated the effects of SRY on cell cycle profiles. It was shown that the over‐expression of SRY negatively regulated the cell growth with significant S or G２／M arrest of cell cycle. |
| Journal Title |
四国医学雑誌
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| ISSN | 00373699
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| NCID | AN00102041
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| Publisher | 徳島医学会
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| Volume | 60
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| Issue | 5-6
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| Start Page | 168
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| End Page | 171
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| Sort Key | 168
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| Published Date | 2004-12-20
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| Remark | |
| EDB ID | |
| FullText File | |
| language |
jpn
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| departments |
Pharmaceutical Sciences
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