| ID | 110758 |
| Author |
Bunpo, Piyawan
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Kataoka, Keiko
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
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Arimochi, Hideki
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
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Nakayama, Haruyuki
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Kuwahara, Tomomi
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Vinitketkumnuen, Usanee
Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Ohnishi, Yoshinari
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
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|
| Keywords | asiatic acid
apoptosis
CPT-11
combination
cytotoxicity
|
| Content Type |
Journal Article
|
| Description | Asiatic acid is a pentacyclic triterpene contained inmedicinal plants. The cytotoxic effect of this compound and its augmentative effect on the anticancer drug irinotecan hydrochloride (CPT-11) were investigated in the human colon adenocarcinoma cell lineHT-29. Asiatic acid dose-dependently showed cytotoxicity in HT-29 cells. DNA fragmentation, annexin-positive apoptotic cells, andcaspase-3 activation were observed in a dose-dependent manner. Acaspase-3 inhibitor suppressed the DNA ladder formation in a concentration-dependent manner. Bcl-2 and Bcl-xL proteins were decreased by asiatic acid treatment. These results indicate that asiatic acid induced apoptosis inHT-29 cells viacaspase-3activation.Cytotoxic effectsof combined treatment with CPT-11 and asiatic acid on HT-29 cells were further examined. Simultaneous treatment or sequential exposure first to asiatic acid and then to CPT-11 showed an additive effect. Synergism was observed when cells were first exposed to CPT-11 and then to asiatic acid. These results suggest that asiatic acid can be used as an agent for increasing sensitivity of colon cancer cells to treatment with CPT-11 or as an agent for reducing adverse effects of CPT-11.
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| Journal Title |
The journal of medical investigation : JMI
|
| ISSN | 13431420
|
| NCID | AA11166929
|
| Volume | 52
|
| Issue | 1-2
|
| Start Page | 65
|
| End Page | 73
|
| Sort Key | 65
|
| Published Date | 2005-02
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| EDB ID | |
| DOI (Published Version) | |
| URL ( Publisher's Version ) | |
| FullText File | |
| language |
eng
|
| TextVersion |
Publisher
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| departments |
Medical Sciences
|
