骨再生エンジニアリングに向けた知的コラーゲン計測・制御手法に関する研究開発

Second-harmonic-generation (SHG) microscopy is a new tool for observing the collagen fiber in tissue in vivo. However, conventional SHG microscopy equipped with 100-fs pulse laser is insufficient to visualize collagen fiber produced by cultured cell with high contrast because of low nonlinear susceptibility x(2) in low-order-structured, immature collagen. To enhance the image contrast while avoiding the sample damage, one has to increase a peak power of the laser light while maintaining the same average power as the 100-fs laser. In this paper, we enhanced the sensitive of SHG microscopy by use of a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in vivo.

この論文の著作権は徳島大学ソシオテクノサイエンス研究部にあります。