ID | 111983 |
Title Alternative | Zinc is a determinant of the cytotoxicity of Ziram, a dithiocarbamate fungicide, in rat thymic lymphocytes : Possible environmental risk
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Author |
Kanemoto-Kataoka, Yumiko
Tokushima University|Bayer Yakuhin, Ltd.
Oyama, Tomohiro M.
Tokushima University|Medical Co. LTA
Ishibashi, Hitoshi
Tokushima University|Kitasato University
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Keywords | Fungicide
Dithiocarbamate
Lymphocytes
Cytotoxicity
Zinc
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Content Type |
Journal Article
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Description | Ziram, one of dithiocarbamate fungicides, is widely applied to agriculture because the agent protects various crops from fungal infections. Risks of dithiocarbamate biocide use are concerned. It was previously reported that Ziram increased the intracellular concentration of Zn2+. Therefore, we cytometrically studied the mechanism of Zn2+-dependent lethal actions of Ziram on rat lymphocytes under environmentally relevant Zn2+ levels. Membrane and cellular parameters of rat lymphocytes were estimated by flow-cytometric techniques with appropriate fluorescent probes. The Ziram-induced increase in cell lethality was completely attenuated by Zn2+ chelators. A significant raise of cell lethality was found on the simultaneous application of Ziram at a sublethal concentration and ZnCl2. The combination of Ziram and ZnCl2 increased the cellular superoxide anion content and decreased the cellular GSH content, which possibly caused the increase in cell lethality. The zinc concentrations under present experimental conditions were comparable to the environmentally relevant concentrations found in rivers. Therefore, the environmental level of zinc may be critical in estimating the toxicity of Ziram to wild animals.
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Journal Title |
Toxicology Research
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ISSN | 20454538
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Publisher | The Royal Society of Chemistry
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Volume | 6
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Issue | 4
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Start Page | 499
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End Page | 504
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Published Date | 2017-04-18
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Rights | ©2017 The Royal Society of Chemistry
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
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language |
eng
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TextVersion |
Author
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departments |
Bioscience and Bioindustry
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