ID | 113270 |
Title Alternative | 子宮内膜症モデルマウスにおいてアクチビンの腹腔内投与は子宮内膜症様病変の増殖を促進する
Activin A promotes endometriotic lesions
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Author |
Kasai, Kana
Tokushima University
Kadota, Yuri
Tokushima University
Erdenebayar, Otgontsetseg
Tokushima University
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Keywords | Endometriosis
Activin A
Mouse model
Inflammatory cytokine
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Content Type |
Thesis or Dissertation
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Description | PURPOSE : This study aimed to investigate the effect of intraperitoneal administration of activin on the occurrence of endometriosis using a mouse model of endometriosis. METHODS : A mouse model of endometriosis was prepared by intraperitoneally administering endometrial tissue and blood collected from donor mice to C57BL/6J 7-8- week-old recipient mice. A total of 400 μg of activin A was intraperitoneally administered to model mice in the activin group for 5 days. Intraperitoneal endometriotic lesions were confirmed macroscopically and IL-6 and TNF-α levels in washed ascites were measured by ELISA. RESULTS : Endometriotic lesions were observed in all mice. In the activin group, the maximum diameter of endometriotic lesions was significantly larger than that in control group (4.7±1.3 vs 2.9±0.9 mm, p<0.01). The total area of the lesion was also significantly higher in the activin group than in the control group (21.1±9.9 vs 8.8±5.4 mm2, p<0.01). Furthermore, IL-6 and TNF-α levels in ascites were significantly higher in the activin group than in the control group (IL-6 : 85.8±15.3 vs 75.1±19.3 pg/ml, p<0.05 ; TNF-α : 629.8±15.4 vs 605.9±11.4 pg/ml, p<0.05). CONCLUSION : Activin promotes occurrence of endometriosis. Inflammatory cytokines are also elevated by activin administration,suggesting that they may contribute to progression of endometriosis
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Journal Title |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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NCID | AA12022913
AA11166929
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Publisher | Tokushima University Faculty of Medicine
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Volume | 66
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Issue | 1-2
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Start Page | 123
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End Page | 127
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Sort Key | 123
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Published Date | 2019-02
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Kana Kasaiの学位論文として提出され,学位審査・授与の対象となっている。 |
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DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3253号
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Diploma Number | 甲医第1402号
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Granted Date | 2019-02-06
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
University Hospital
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