ID | 113896 |
Author |
Sakamoto, Takaiku
Tokushima University
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Sakuradani, Eiji
Tokushima University
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Okuda, Tomoyo
Kyoto University
Kikukawa, Hiroshi
Gifu University
Ando, Akinori
Kyoto University
Kishino, Shigenobu
Kyoto University
Izumi, Yoshihiro
Kyushu University
Bamba, Takeshi
Kyushu University
Shima, Jun
Ryukoku University
Ogawa, Jun
Kyoto University
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Keywords | Mortierella alpina
Oleic acid
Linoleic acid
Fatty acid desaturase
RNAi
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Content Type |
Journal Article
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Description | The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
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Journal Title |
Bioresource Technology
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ISSN | 18732976
09608524
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NCID | AA11522750
AA10838373
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Publisher | Elsevier
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Volume | 245
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Issue | B
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Start Page | 1610
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End Page | 1615
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Published Date | 2017-06-19
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Rights | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
Author
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departments |
Bioscience and Bioindustry
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