| ID | 114845 |
| Author |
Mine, Masanori
Tokushima University
|
| Keywords | Capillary electrophoresis
Alkaline phosphatase
Inhibition assay
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| Content Type |
Journal Article
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| Description | A novel inhibition assay is proposed by capillary electrophoresis/dynamic frontal analysis (CE/DFA). When a substrate of p-nitrophenyl phosphate and an inhibitor of theophylline were tandemly introduced into the capillary containing alkaline phosphatase as an enzyme, two plateau signals were detected in the electropherogram. A higher plateau is based on the CE/DFA without inhibition, and a suppressed plateau is formed under the inhibition while the substrate zone passing through the inhibitor zone. Inhibition constant was successfully determined through the two plateau heights.
|
| Journal Title |
Chemistry Letters
|
| ISSN | 13480715
|
| NCID | AA12179675
|
| Publisher | The Chemical Society of Japan
|
| Volume | 49
|
| Issue | 6
|
| Start Page | 681
|
| End Page | 684
|
| Published Date | 2020-03-26
|
| Rights | © 2020 The Chemical Society of Japan
|
| EDB ID | |
| DOI (Published Version) | |
| URL ( Publisher's Version ) | |
| FullText File | |
| language |
eng
|
| TextVersion |
Author
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| departments |
Science and Technology
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