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ID 116063
Title Alternative
血管内皮細胞における過酸化水素誘導のカベオリン-1リン酸化に対するケルセチンの抑制効果
Author
Kondo-Kawai, Akari Tokushima University|Shikoku Junior College
Keywords
quercetin
caveolae
caveolin-1
hydrogen peroxide
vascular endothelium
Content Type
Thesis or Dissertation
Description
Caveolin-1 is a major protein of the caveolae structure in vascular endothelial cell membrane. Phosphorylation of caveolin-1 is one of the initial events leading to exacerbation of vascular permeability caused by oxidative stress. Although quercetin is known to be an anti-atherosclerosis factor that acts as a dietary antioxidant, little is known about its role in the regulation of caveolin-1 phosphorylation. In this study, we investigated the inhibitory effect of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in human umbilical vein endothelial cells. Quercetin inhibited caveolin-1 phosphorylation in cells pretreated with quercetin for 24 h and then exposed to hydrogen peroxide. However, quercetin 3-O-β-glucuronide, a conjugated metabolite of quercetin, did not exert this inhibitory effect. Exposure to hydrogen peroxide increased vascular permeability and reduced mRNA expression of the intercellular adhesion protein, vascular endothelial cadherin (VE-cadherin). By contrast, pretreatment with quercetin suppressed the increase in vascular permeability and decreased VE-cadherin expression. These results indicate that deconjugated quercetin can play a role in the prevention of altered vascular permeability under oxidative stress by suppressing caveolin-1 phosphorylation. Thus, dietary quercetin may be beneficial for the maintenance of endothelial cell function.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
NCID
AA10710201
Publisher
Society for Free Radical Research Japan
Volume
69
Issue
1
Start Page
28
End Page
36
Published Date
2021-07-01
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Akari Kondo-Kawaiの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2020 Society for Free Radical Research Japan
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License. https://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3510号
Diploma Number
甲栄第284号
Granted Date
2021-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
Bioscience and Bioindustry