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ID 116383
Title Alternative
片側末梢投与されたA型ボツリヌス毒素は動物モデルにおいて両側三叉神経節に局在する
Author
Waskitho, Arief Tokushima University
Yamamoto, Yumiko Okayama University
Raman, Swarnalakshmi Tokushima University
Yan, Huijiao Tokushima University
Raju, Resmi National Center for Geriatrics and Gerontology
Afroz, Shaista Aligarh Muslim University
Keywords
Botulinum toxin
trigeminal ganglion
neuropathic pain
Content Type
Thesis or Dissertation
Description
Peripheral nerve injury leads to sensory ganglion hyperexcitation, which increases neurotransmitter release and neuropathic pain. Botulinum toxin type A (BoNT/A) regulates pain transmission by reducing neurotransmitter release, thereby attenuating neuropathic pain. Despite multiple studies on the use of BoNT/A for managing neuropathic pain in the orofacial region, its exact mechanism of transport remains unclear. In this study, we investigated the effects of BoNT/A in managing neuropathic pain in two different animal models and its transport mechanism in the trigeminal nerve. Intraperitoneal administration of cisplatin induced bilateral neuropathic pain in the orofacial region, reducing the head withdrawal threshold to mechanical stimulation. Unilateral infraorbital nerve constriction (IONC) also reduced the ipsilateral head withdrawal threshold to mechanical stimulation. Unilateral peripheral administration of BoNT/A to the rat whisker pad attenuated cisplatin-induced pain behavior bilaterally. Furthermore, contralateral peripheral administration of BoNT/A attenuated neuropathy-induced behavior caused by IONC. We also noted the presence of BoNT/A in the blood using the mouse bioassay. In addition, the Alexa Fluor-488-labeled C-terminal half of the heavy chain of BoNT/A (BoNT/A-Hc) was localized in the neurons of the bilateral trigeminal ganglia following its unilateral administration. These findings suggest that axonal and hematogenous transport are involved in the therapeutic effects of peripherally administered BoNT/A in the orofacial region.
Journal Title
Toxins
ISSN
20726651
Publisher
MDPI
Volume
13
Issue
10
Start Page
704
Published Date
2021-10-05
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Arief Waskithoの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3609号
Diploma Number
甲口第477号
Granted Date
2022-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
University Hospital