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ID 116865
Title Alternative
部分的最小二乗法によるFIAの迅速化 : フェナントロリン吸光光度法を用いる鉄の定量による検証
Rapid Flow Injection Analysis by Partial Least Squares Regression : Validation by a Spectrophotometric Determination of Iron with 1,10-Phenanthroline
Author
Takahashi, Toru Tokushima University
Watanabe, Mayu Tokushima University
Otsuka, Yuta Tokyo University of Science
Keywords
FIA
overlapped peak
partial least squares regression
rapid analysis
Content Type
Journal Article
Description
フローインジェクション分析法(FIA)では,試料の導入間隔を短くするとスループットは向上するが,試料の分散によるピークの重なりが起こり,分析の精確さが低下する.本研究では,重なりピークに部分的最小二乗法(PLS)を適用することで,目的成分を精確かつ迅速に定量可能なFIA,すなわちRFP(Rapid FIA by PLS regression)法の確立を目指した.フェナントロリン吸光光度法によるFe2+のFIAをRFP法の評価モデルとした.二つのFe2+試料(S1, S2)を一定間隔で導入し,それぞれのピークが重なっている吸光度シグナルを得た.得られたシグナルにPLSを適用してPLS予測検量線を作成したところ,S1とS2の検量線の傾きはいずれもほぼ1であり,直線性も良好であった.環境水に含まれるFe2+をRFP法と絶対検量線法(完全分離したピークの高さから濃度を算出)でそれぞれ定量した結果,両者の分析値の間に有意な差はなく(p>0.05),RFP法の導入によりスループットを絶対検量線法の約1.7倍に高めることができた.以上の結果から,PLSを導入したFIA,すなわちREP法は重なった吸光度ピークでも精確な定量が可能であり,試料導入間隔の短縮による分析の迅速化に有効であると結論した.
Description Alternative
We applied a partial least squares regression (PLS) to overlapped peaks to develop a rapid flow injection analysis by the PLS method (RFP method) that quantifies the analytes of interest both accurately and rapidly. Spectrophotometric determination of Fe2+ with 1,10-phenanthroline was used as an evaluation model of the RFP method. Two Fe2+ standard samples (S1 and S2) were sequentially injected at a 30-sec interval, and the overlapped peaks were obtained. A calibration curve was constructed by applying the PLS to the overlapped peaks. The slopes of the calibration curve for S1 and S2 were both almost unity with good linearity. The concentrations of Fe2+ in surface water samples were determined by the RFP method. The Fe2+ concentrations measured were in good agreement with the values calculated by applying an absolute calibration curve method to non-overlapped peaks (ACC method). The sample throughput of the RFP method was about 1.7-times faster than that of the ACC method. We concluded that the RFP method achieves both accurate and rapid quantification.
Journal Title
BUNSEKI KAGAKU
ISSN
05251931
NCID
AN00222633
Publisher
日本分析化学会
Volume
70
Issue
7-8
Start Page
451
End Page
457
Published Date
2021-07-05
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
jpn
TextVersion
Publisher
departments
Pharmaceutical Sciences