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ID 117138
Title Alternative
Novel method for detection of pancreatic beta cell death using cell-free DNA
Author
Okada, Asami Tokushima University
Yamada, Misuzu Tokushima University
Akehi, Yuko Tokushima University
Keywords
Insulin
DNA methylation
RT-PCR
Type1 diabetes
cell-free DNA
Content Type
Journal Article
Description
In people with type1 diabetes(T1D), biomarkers that can sensitively and quantitatively evaluate injury of pancreatic beta cell are required in order to predict the onset of the disease at an early stage and to provide interventions to prevent the progression of the disease. We developed a new method for quantifying pancreatic beta cell-derived insulin DNA in circulation that combines bisulfite conversion and Amplification Refractory Mutation System(ARMS)PCR, which can be performed using a conventional real-time PCR system. We applied this method to T1D patients and healthy adults, both could be detected in about 30% of cases. The results in healthy adults indicate that this method may have sensitivity to detect the turnover of pancreatic beta cells at physiological conditions. In post-onset T1D patients, there were many negatives because the amount of residual pancreatic beta cells was extremely small. However, in some cases with a short duration of the disease, pancreatic beta cell-derived insulin DNA was detected in negative correlation between the duration of the disease, that suggested the residual pancreatic beta cells continue to be slowly destroyed. It was demonstrated that the time course of pathophysiology in T1D could be understood using this method.
Journal Title
Shikoku Acta Medica
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
77
Issue
5-6
Start Page
249
End Page
254
Sort Key
249
Published Date
2021-12-25
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Institute of Advanced Medical Sciences
University Hospital
Medical Sciences