ID 101801
Title Transcription
セイブンカ ト セイシ ケイセイ キコウ ニ タイスル プロテオミクス カラノ アプローチ
Title Alternative
Proteomics approach for the mechanism of sex determination
Author
Sato, Youichi Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Shinka, Toshikatsu Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Chen, Gang Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Yan, Hong-Tao Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Sakamoto, Kozue Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Yang, Xin‐Jun Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Umeno, Mayumi School of Health Science, The University of Tokushima Faculty of Medicine
Nakahori, Yutaka Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School
Keywords
SRY
sex determination
Y chromosome
proteomics
Content Type
Journal Article
Description
SRY (sex determining region on the Y chromosome) was a gene isolated as the testis determining factor on the Y chromosome in 1990.The gene consisted of single exon and encodes a 204 amino acid protein. Since SRY protein has a HMG domain, which functions as DNA binding domain, it has been considered to be a transcription factor. Direct target of SRY, however, have not yet identified for 14 years.
In this study, we have performed a proteomics approach to analyze the function of SRY. Twodimensional gel electrophoresis after over expression of SRY showed considerable down regulation in many proteins. Among the proteins, mitochondrial 60‐kDa heat shock protein (HSP60) and probable protein disulfide isomerase (ER60) remarkably decreased. Since they were reported to be associated with cell proliferation and differentiation, we investigated the effects of SRY on cell cycle profiles. It was shown that the over‐expression of SRY negatively regulated the cell growth with significant S or G2/M arrest of cell cycle.
Journal Title
四国医学雑誌
ISSN
00373699
NCID
AN00102041
Publisher
徳島医学会
Volume
60
Issue
5-6
Start Page
168
End Page
171
Sort Key
168
Published Date
2004-12-20
Remark
EDB ID
FullText File
language
jpn
departments
Pharmaceutical Sciences