ID 104978
Author
Fukunaga, Eri Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
Kanbara, Yasuhiro Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
Hirao, Yumi Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
Oyama, Yasuo Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Intracellular Zn2+
Chemical stress
Intracellular thiol
Triclocarban
Content Type
Departmental Bulletin Paper
Description
We have proposed that intracellular Zn2+ release during oxidative stress is a trigger to restore cellular thiol content that is decreased by oxidative stress. Recently, we found that the incubation with triclocarban for 1 h decreased cellular thiol content and increased intracellular Zn2+ concentration. It was reminiscent of the possibility that the increase in intracellular Zn2+ concentration by triclocarban could become a trigger to restore the cellular content of nonprotein thiols. To test the possibility, we cytometrically examined the effects of prolonged incubation (3 h) with triclocarban on the cellular content of nonprotein thiols, presumably glutathione, by the use of 5-chloromethylfluorescein (5-CMF) diacetate, a fluorescent indicator of cellular nonprotein thiols, in rat thymocytes. The intensity of 5-CMF fluorescence after the 3 h incubation with 300 nM triclocarban was significantly higher than that after the 1 h incubation. In the presence of a chelator of intracellular Zn2+, such a significant difference was not observed. The results suggest that the increase in intracellular Zn2+ concentration by triclocarban is one of triggers to restore cellular content that is decreased by triclocarban.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
27
Issue
1
Start Page
1
End Page
5
Sort Key
1
Published Date
2013-01
EDB ID
FullText File
language
eng
departments
Bioscience and Bioindustry