ID 104978
著者
フクナガ, エリ Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
カンバラ, ヤスヒロ Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
ヒラオ, ユミ Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
小山, 保夫 Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Intracellular Zn2+
Chemical stress
Intracellular thiol
Triclocarban
資料タイプ
紀要論文
抄録
We have proposed that intracellular Zn2+ release during oxidative stress is a trigger to restore cellular thiol content that is decreased by oxidative stress. Recently, we found that the incubation with triclocarban for 1 h decreased cellular thiol content and increased intracellular Zn2+ concentration. It was reminiscent of the possibility that the increase in intracellular Zn2+ concentration by triclocarban could become a trigger to restore the cellular content of nonprotein thiols. To test the possibility, we cytometrically examined the effects of prolonged incubation (3 h) with triclocarban on the cellular content of nonprotein thiols, presumably glutathione, by the use of 5-chloromethylfluorescein (5-CMF) diacetate, a fluorescent indicator of cellular nonprotein thiols, in rat thymocytes. The intensity of 5-CMF fluorescence after the 3 h incubation with 300 nM triclocarban was significantly higher than that after the 1 h incubation. In the presence of a chelator of intracellular Zn2+, such a significant difference was not observed. The results suggest that the increase in intracellular Zn2+ concentration by triclocarban is one of triggers to restore cellular content that is decreased by triclocarban.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
27
1
開始ページ
1
終了ページ
5
並び順
1
発行日
2013-01
EDB ID
258784
フルテキストファイル
言語
eng
部局
生物資源系