ID 105963
Title Transcription
アミトロール テイコウセイ ダイチョウキン カブ ニオケル タンパクシツ ハツゲン カイセキ
Title Alternative
Protein Expression Analysis in Amitrole-Resistant E.coli Strains
Author
Yamaguchi, Yasuaki Faculty of Integrated Arts and Sciences, The University of Tokushima
Miyano-Ono, Momoyo Faculty of Integrated Arts and Sciences, The University of Tokushima
Kyogoku-Tsujinaka, Hitomi Faculty of Integrated Arts and Sciences, The University of Tokushima
Yamaguchi, Ayano Faculty of Integrated Arts and Sciences, The University of Tokushima
Aoki, Taku Graduate school of Human and Natural Environment Sciences, The University of Tokushima
Satoh, Takanori Institute of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Amitrole
Adaptation
Expression
E.coli
Chemical mutagen
Content Type
Departmental Bulletin Paper
Description
It was reported that the E.coli mutant strain exhibited the resistance against amitrole (AT) which had used as a pesticide and inhibits the bacterial growth. In order to elucidate the mechanism on the AT-resistance in E.coli, we prepared the E.coli mutant strain by treating with chemical mutagen, MNNG, and then selected the AT-resistant E. coli strain. The obtained mutant strain was possible for proliferating even in the M9 minimal medium containing 2 mg/ml AT. Therefore, we examined the expressed proteins in the mutant strain, which was cultured under various conditions. As a result, it was suggested the expression of 22 kDa protein (P1) was suppressed as the AT concentration was increased in M9 medium. By the amino acid sequence analysis, it was proved that P1 must be alkyl hydroperoxide reductase C22 protein (ahpC). On the other hand, the expression of P2 protein in mutant strain has increased in the M9 medium containing 2mg/ml AT, and partial amino acid sequence of P2 was consistent with zinT (yodA) protein. From these results, it is likely that the amitrole-resistance in E.coli might be regulated by soxRS regulon, not oxy R, like adaptation for the oxidative stress.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
27
Issue
4
Start Page
81
End Page
88
Sort Key
81
Published Date
2013-10
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Science and Technology