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ID 106315
Title Alternative
YbはpiRNA前駆体とpiRNA生合成因子を核周辺部位に形成される顆粒体に局在させることによってpiRISC形成を促進する
Author
Murota, Yukiko Institute for Genome Research, University of Tokushima|Keio University
Ishizu, Hirotsugu The University of Tokyo
Nakagawa, Shinichi RIKEN
Iwasaki, Yuka W. Keio University
Shibata, Shinsuke Keio University
Kamatani, Miharu K. Keio University
Saito, Kuniaki Keio University
Okano, Hideyuki Keio University
Shiomi, Haruhiko Keio University
Siomi, Mikiko C. The University of Tokyo
Keywords
piRNA
Piwi
flamenco
Yb body
Drosophila
Content Type
Thesis or Dissertation
Description
PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable for formation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
Journal Title
Cell Reports
ISSN
22111247
Publisher
Elsevier
Volume
8
Issue
1
Start Page
103
End Page
113
Published Date
2014-06-19
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201411041003.pdf
論文本文 : LID201411041004.pdf
本論文は, 著者Yukiko Murotaの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2779号
Diploma Number
甲医第1224号
Granted Date
2014-10-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University