ID 106315
Title Alternative
YbはpiRNA前駆体とpiRNA生合成因子を核周辺部位に形成される顆粒体に局在させることによってpiRISC形成を促進する
Author
Murota, Yukiko Institute for Genome Research, University of Tokushima
Content Type
Thesis or Dissertation
Description
PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable for formation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
Published Date
2014-10-23
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201411041003.pdf
論文本文 : LID201411041004.pdf
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
Published Source
Cell Reports (2014) Vol.8 Issue.1 p.103-113 (DOI: 10.1016/j.celrep.2014.05.043)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2779号
Diploma Number
甲医第1224号
Granted Date
2014-10-23
Degree Name
Doctor of Medical Science
Grantor
Tokushima University