ID 106315
タイトル別表記
YbはpiRNA前駆体とpiRNA生合成因子を核周辺部位に形成される顆粒体に局在させることによってpiRISC形成を促進する
著者
室田, 友紀子 徳島大学大学院医科学教育部(医学専攻)
資料タイプ
学位論文
抄録
PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable for formation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
発行日
2014-10-23
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201411041003.pdf
論文本文 : LID201411041004.pdf
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
出典
Cell Reports (2014) Vol.8 Issue.1 p.103-113 (DOI: 10.1016/j.celrep.2014.05.043)
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2779号
学位記番号
甲医第1224号
学位授与年月日
2014-10-23
学位名
博士(医学)
学位授与機関
徳島大学