ID 106357
Author
Jambaldorj, Bayasgalan Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Terada, Eri Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Hosaka, Toshio Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Kishuku, Yuka Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Tomioka, Yukiko Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Iwashima, Kaori Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Hirata, Yohko Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Teshigawara, Kiyoshi Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Chung, Thi Kim Le Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School
Nakagawa, Tadahiko Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Harada, Nagakatsu Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Sakai, Tohru Department of Public Health and Applied Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sakaue, Hiroshi Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Matsumoto, Toshio Department of Medicine and Bioregulatory Sciences, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Funaki, Makoto Clinical Research Center for Diabetes, Tokushima University Hospital Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takahashi, Akira Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nakaya, Yutaka Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
GLUT4
cysteine string protein 1
SNARE
VAMP2
Content Type
Journal Article
Description
Insulin stimulates glucose transporter 4 (GLUT4) vesicle recruitment from its intracellular storage site to the plasma membrane. Cysteine string protein 1 (CSP1) is a SNARE-binding protein involved in the vesicular trafficking of neurotransmitters and other exocytic processes. In this study, we investigated the involvement of CSP1 in insulin-dependent GLUT4 recruitment in 3T3-L1 adipocytes. Over-expression of wild-type CSP1 led to attenuated insulin-stimulated glucose uptake without any change in GLUT4 content in the plasma membrane, rather it inhibits docking by blocking the association of VAMP2 with syntaxin 4. In contrast, knockdown of CSP1 enhanced insulin-stimulated glucose uptake. The mRNA and protein expression of CSP1 was elevated in 3T3-L1 adipocytes in insulin resistant states caused by high levels of palmitate and chronic insulin exposure. Taken together, the results of this study suggest that CSP1 is involved in insulin resistance by interrupting GLUT4 vesicle docking with the plasma membrane.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
60
Issue
3-4
Start Page
197
End Page
204
Sort Key
197
Published Date
2013-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences
University Hospital
Institute of Advanced Medical Sciences