ID 106381
Title Transcription
モンゴルコク カチクニュウ オヨビ ニュウセイヒンチュウ ノ サイキン ノ クローン ライブラリーホウ ニヨル カイセキ
Title Alternative
Phytogenetic analysis of bacteria from Mongolian animal milks and their dairy products by clone library method
Author
Oe, Yuto Laboratory of Biochemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima
Abe, Momoko Laboratory of Biochemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima
Endoh, Yousuke Laboratory of Biochemistry,Graduate school of Integrated Arts and Sciences, The University of Tokushima
Sakata, Masahiro Laboratory of Biochemistry,Graduate school of Integrated Arts and Sciences, The University of Tokushima
Ichinkhorloo, Zesem School of Biomedicine, Health Science University of Mongolia
Munkhtsetseg, Janlav School of Biomedicine, Health Science University of Mongolia
Ohashi, Makoto Institute of Socio-Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Satoh, Takanori Institute of Socio-Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Animal milk
Dairy product
Clone library
16S rRNA gene
Mongolia
Content Type
Departmental Bulletin Paper
Description
Various animal milks and their dairy products like AIRAG (fermented horse milk) and yogurt are very popular among Mongolian people. The climate of Mongolia is very severe, and they preserve their health by the intake of above animal milks and dairy products. In this study, we explored the diversity of bacteria in Mongolian animal milks (Cow, Horse, Goat, and Camel), AIRAG, and Camel milk yogurt by the clone library method of their 16S rRNA genes.
Firstly, we prepared the whole genomic DNA from four animal milks and two dairy products, and amplified each 16S rRNA genes by PCR. PCR products (about 1.4kbp) were cloned into pGEM-T vector, and analyzed DNA sequences of totally 79 clones from AIRAG, Camel milk yogurt, and four animal milks. It was revealed that homologous clones to Lactobacillus helveticus are dominant in the clone libraries of both dairy products, whereas the other clones to Lactococcus from AIRAG, and Acetobacter from camel milk yogurt. Furthermore, We found that homologous clones to Lactococcus are dominant in Mongolian cow and horse milks, whereas Leuconostoc in camel milk. Therefore, it might be useful information for screening the bioactive strains from milk products in Mongolia.
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
28
Issue
4
Start Page
27
End Page
36
Sort Key
27
Published Date
2014-10
FullText File
language
jpn
TextVersion
Publisher
departments
Liberal Arts and Sciences
Science and Technology