モンゴルコク カチクニュウ オヨビ ニュウセイヒンチュウ ノ サイキン ノ クローン ライブラリーホウ ニヨル カイセキ
Phytogenetic analysis of bacteria from Mongolian animal milks and their dairy products by clone library method
麻植, 優人 徳島大学総合科学部社会創生学科
阿部, 桃子 徳島大学総合科学部社会創生学科
遠藤, 陽介 徳島大学大学院総合科学教育部地域科学専攻
坂田, 真宏 徳島大学大学院総合科学教育部地域科学専攻
Ichinkhorloo, Zesem School of Biomedicine, Health Science University of Mongolia
Munkhtsetseg, Janlav School of Biomedicine, Health Science University of Mongolia
16S rRNA gene
Various animal milks and their dairy products like AIRAG (fermented horse milk) and yogurt are very popular among Mongolian people. The climate of Mongolia is very severe, and they preserve their health by the intake of above animal milks and dairy products. In this study, we explored the diversity of bacteria in Mongolian animal milks (Cow, Horse, Goat, and Camel), AIRAG, and Camel milk yogurt by the clone library method of their 16S rRNA genes.
Firstly, we prepared the whole genomic DNA from four animal milks and two dairy products, and amplified each 16S rRNA genes by PCR. PCR products (about 1.4kbp) were cloned into pGEM-T vector, and analyzed DNA sequences of totally 79 clones from AIRAG, Camel milk yogurt, and four animal milks. It was revealed that homologous clones to Lactobacillus helveticus are dominant in the clone libraries of both dairy products, whereas the other clones to Lactococcus from AIRAG, and Acetobacter from camel milk yogurt. Furthermore, We found that homologous clones to Lactococcus are dominant in Mongolian cow and horse milks, whereas Leuconostoc in camel milk. Therefore, it might be useful information for screening the bioactive strains from milk products in Mongolia.
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
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